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LSD2/KDM1B/AOF1 Rabbit mAb (A19820)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LSD2/KDM1B/AOF1 Rabbit mAb (A19820)

Western blot analysis of extracts of various cell lines, using LSD2/AOF1 antibody (A19820) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameLSD2/KDM1B/AOF1 Rabbit mAb
Catalog No.A19820
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2347
Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 [PubMed 19407342]).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human LSD2/KDM1B/AOF1 (NP_694587.3).
SequenceMATPRGRTKKKASFDHSPDSLPLRSSGRQAKKKATETTDEDEDGGSEKKYRKCEKAGCTATCPVCFASASERCAKNGYTSRWYHLSCGEHFCNECFDHYY
Gene ID
Swiss Prot
SynonymsAOF1; LSD2; C6orf193
Calculated MW92kDa
Observed MW92kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, HCT 116, HepG2
Cellular locationnucleoplasm, nucleus
Customer validation

IF(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - LSD2/KDM1B/AOF1 Rabbit mAb (A19820)}

    Western blot - LSD2/KDM1B/AOF1 Rabbit mAb (A19820)

    Western blot analysis of extracts of various cell lines, using LSD2/AOF1 antibody (A19820) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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