제품 > 항체 > 다중클론항체(pAb)

LTF Rabbit pAb (A12902)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LTF Rabbit pAb (A12902)

Western blot analysis of lysates from Rat lung, using LTF Rabbit pAb (A12902) at 1:1500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - LTF Rabbit pAb (A12902)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using LTF Rabbit pAb (A12902) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - LTF Rabbit pAb (A12902)

Immunohistochemistry analysis of paraffin-embedded Human placenta using LTF Rabbit pAb (A12902) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - LTF Rabbit pAb (A12902)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using LTF Rabbit pAb (A12902) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameLTF Rabbit pAb
Catalog No.A12902
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Activity against both DNA and RNA viruses has been found, including activity against SARS-CoV-2, and HIV.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 21-352 of human LTF (NP_002334.2).
SequenceRRRSVQWCAVSQPEATKCFQWQRNMRKVRGPPVSCIKRDSPIQCIQAIAENRADAVTLDGGFIYEAGLAPYKLRPVAAEVYGTERQPRTHYYAVAVVKKGGSFQLNELQGLKSCHTGLRRTAGWNVPIGTLRPFLNWTGPPEPIEAAVARFFSASCVPGADKGQFPNLCRLCAGTGENKCAFSSQEPYFSYSGAFKCLRDGAGDVAFIRESTVFEDLSDEAERDEYELLCPDNTRKPVDKFKDCHLARVPSHAVVARSVNGKEDAIWNLLRQAQEKFGKDKSPKFQLFGSPSGQKDLLFKDSAIGFSRVPPRIDSGLYLGSGYFTAIQNLRK
Gene ID
Swiss Prot
SynonymsLF; HLF2; GIG12; HEL110; LTF
Calculated MW78kDa
Observed MW85kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRat lung
Cellular locationCytoplasm, Cytoplasmic granule, Nucleus, Secreted.
Customer validation

WB(Mus musculus)

IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - LTF Rabbit pAb (A12902)}

Western blot - LTF Rabbit pAb (A12902)

Western blot analysis of lysates from Rat lung, using LTF Rabbit pAb (A12902) at 1:1500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - LTF Rabbit pAb (A12902)}

Immunohistochemistry - LTF Rabbit pAb (A12902)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using LTF Rabbit pAb (A12902) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - LTF Rabbit pAb (A12902)}

Immunohistochemistry - LTF Rabbit pAb (A12902)

Immunohistochemistry analysis of paraffin-embedded Human placenta using LTF Rabbit pAb (A12902) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - LTF Rabbit pAb (A12902)}

Immunohistochemistry - LTF Rabbit pAb (A12902)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using LTF Rabbit pAb (A12902) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A12902? Please let us know so that we can cite the reference in this datasheet.

ELISA 키트 (2)

재조합 단백질 (1)

Secondary Antibodies (26)