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Lactate Dehydrogenase C Rabbit mAb (A20866)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Lactate Dehydrogenase C Rabbit mAb (A20866)

Western blot analysis of extracts of various cell lines, using Lactate Dehydrogenase C antibody (A20866) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameLactate Dehydrogenase C Rabbit mAb
Catalog No.A20866
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2796
Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Lactate Dehydrogenase C (P07864).
SequenceMSTVKEQLIEKLIEDDENSQCKITIVGTGAVGMACAISILLKDLADELALVDVALDKLKGEMMDLQHGSLFFSTSKITSGKDYSVSANSRIVIVTAGARQ
Gene ID
Swiss Prot
SynonymsCT32; LDH3; LDHX
Calculated MW36kDa
Observed MW36kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular locationCytoplasm

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ABclonal:Western blot - Lactate Dehydrogenase C Rabbit mAb (A20866)}

Western blot - Lactate Dehydrogenase C Rabbit mAb (A20866)

Western blot analysis of extracts of various cell lines, using Lactate Dehydrogenase C antibody (A20866) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)