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Lamin B2 Rabbit mAb (A5001)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Lamin B2 Rabbit mAb (A5001)

Western blot analysis of various lysates using Lamin B2 Rabbit mAb (A5001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Rat brain using Lamin B2 Rabbit mAb (A5001) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Lamin B2 Rabbit mAb (A5001) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using Lamin B2 Rabbit mAb (A5001) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Lamin B2 Rabbit mAb (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Lamin B2 Rabbit mAb (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Lamin B2 Rabbit mAb (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Lamin B2 Rabbit mAb (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Lamin B2 Rabbit mAb (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Lamin B2 Rabbit mAb (A5001)

Confocal imaging of HeLa cells using Lamin B2  Rabbit mAb (A5001, dilution 1:100) (Red). The cells were counterstained with α-Tubulin Rabbit mAb (AC049, dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Overview

Product nameLamin B2 Rabbit mAb
Catalog No.A5001
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1252
This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 521-620 of human Lamin B2 (Q03252).
SequenceYILRAGQMVTVWAAGAGVAHSPPSTLVWKGQSSWGTGESFRTVLVNADGEEVAMRTVKKSSVMRENENGEEEEEEAEFGEEDLFHQQGDPRTTSRGCYVM
Gene ID
Swiss Prot
SynonymsEPM9; LMN2; LAMB2; MCPH27; Lamin B2
Calculated MW70kDa
Observed MW68kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, RD, Jurkat
Cellular locationLipid-anchor, Nucleoplasmic side, Nucleus inner membrane
Customer validation

IF(Gallus gallus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Lamin B2 Rabbit mAb (A5001)}

Western blot - Lamin B2 Rabbit mAb (A5001)

Western blot analysis of various lysates using Lamin B2 Rabbit mAb (A5001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)}

Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Rat brain using Lamin B2 Rabbit mAb (A5001) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)}

Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Lamin B2 Rabbit mAb (A5001) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)}

Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using Lamin B2 Rabbit mAb (A5001) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)}

Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Lamin B2 Rabbit mAb (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)}

Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Lamin B2 Rabbit mAb (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)}

Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Lamin B2 Rabbit mAb (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)}

Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Lamin B2 Rabbit mAb (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)}

Immunohistochemistry - Lamin B2 Rabbit mAb (A5001)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Lamin B2 Rabbit mAb (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Lamin B2 Rabbit mAb (A5001)}

Immunofluorescence - Lamin B2 Rabbit mAb (A5001)

Confocal imaging of HeLa cells using Lamin B2  Rabbit mAb (A5001, dilution 1:100) (Red). The cells were counterstained with α-Tubulin Rabbit mAb (AC049, dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)