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Ly-6G Rabbit mAb (A22270)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - Ly-6G Rabbit mAb (A22270)

Western blot analysis of various lysates using Ly-6G Rabbit mAb (A22270) at 1:11000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): NIH/3T3
Exposure time: 45s.

ABclonal:Immunohistochemistry - Ly-6G Rabbit mAb (A22270)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Ly-6G Rabbit mAb (A22270) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Ly-6G Rabbit mAb (A22270)

Confocal imaging of paraffin-embedded Mouse spleen tissue using Ly-6G Rabbit mAb (A22270, dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - Ly-6G Rabbit mAb (A22270)

Confocal imaging of paraffin-embedded Mouse lung tissue using Ly-6G Rabbit mAb (A22270, dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameLy-6G Rabbit mAb
Catalog No.A22270
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55430
Located in external side of plasma membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 27-105 of mouse Ly-6G (NP_001297367.1).
SequenceLECYNCIGVPPETSCNTTTCPFSDGFCVALEIEVIVDSHRSKVKSNLCLPICPTTLDNTEITGNAVNVKTYCCKEDLCN
Gene ID
Swiss Prot
SynonymsGr1; Gr-1; Ly-6G
Calculated MW14kDa
Observed MW20-40kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5500 - 1:22000
  • IHC-P 1:2000 - 1:8000
  • IF/ICC 1:400-1:1600
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azid, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse bone marrow
Cellular locationexternal side of plasma membrane.
Customer validation

IHC(Homo sapiens, Mus musculus)

IF(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Ly-6G Rabbit mAb (A22270)}

    Western blot - Ly-6G Rabbit mAb (A22270)

    Western blot analysis of various lysates using Ly-6G Rabbit mAb (A22270) at 1:11000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Negative control (NC): NIH/3T3
    Exposure time: 45s.
    ABclonal:Immunohistochemistry - Ly-6G Rabbit mAb (A22270)}

    Immunohistochemistry - Ly-6G Rabbit mAb (A22270)

    Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Ly-6G Rabbit mAb (A22270) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Ly-6G Rabbit mAb (A22270)}

    Immunofluorescence - Ly-6G Rabbit mAb (A22270)

    Confocal imaging of paraffin-embedded Mouse spleen tissue using Ly-6G Rabbit mAb (A22270, dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
    ABclonal:Immunofluorescence - Ly-6G Rabbit mAb (A22270)}

    Immunofluorescence - Ly-6G Rabbit mAb (A22270)

    Confocal imaging of paraffin-embedded Mouse lung tissue using Ly-6G Rabbit mAb (A22270, dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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