제품 > 항체 > 다중클론항체(pAb)

Ly-6G Rabbit pAb (A20861)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - Ly-6G Rabbit pAb (A20861)

Western blot analysis of Mouse bone marrow, using Ly-6G Rabbit pAb (A20861) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - Ly-6G Rabbit pAb (A20861)

Immunofluorescence analysis of mouse spleen cells using Ly-6G Rabbit pAb (A20861) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameLy-6G Rabbit pAb
Catalog No.A20861
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Located in external side of plasma membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 27-105 of mouse Ly-6G (NP_001297367.1).
SequenceLECYNCIGVPPETSCNTTTCPFSDGFCVALEIEVIVDSHRSKVKSNLCLPICPTTLDNTEITGNAVNVKTYCCKEDLCN
Gene ID
Swiss Prot
SynonymsGr1; Gr-1; Ly-6G
Calculated MW14kDa
Observed MW20-40kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse bone marrow
Cellular locationexternal side of plasma membrane
Customer validation

IF(Mus musculus, Anatinae)

WB(Mus musculus)

IHC(Anatinae,Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Ly-6G Rabbit pAb (A20861)}

    Western blot - Ly-6G Rabbit pAb (A20861)

    Western blot analysis of Mouse bone marrow, using Ly-6G Rabbit pAb (A20861) at 1:400 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunofluorescence - Ly-6G Rabbit pAb (A20861)}

    Immunofluorescence - Ly-6G Rabbit pAb (A20861)

    Immunofluorescence analysis of mouse spleen cells using Ly-6G Rabbit pAb (A20861) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20861? Please let us know so that we can cite the reference in this datasheet.