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LysRS/KARS Rabbit mAb (A8648)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LysRS/KARS Rabbit mAb (A8648)

Western blot analysis of various lysates using LysRS/KARS Rabbit mAb (A8648) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - LysRS/KARS Rabbit mAb (A8648)

Immunohistochemistry analysis of paraffin-embedded Rat testis using LysRS/KARS Rabbit mAb (A8648) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LysRS/KARS Rabbit mAb (A8648)

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using LysRS/KARS Rabbit mAb (A8648) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - LysRS/KARS Rabbit mAb (A8648)

Immunofluorescence analysis of C6 cells using LysRS/KARS Rabbit mAb (A8648) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LysRS/KARS Rabbit mAb (A8648)

Immunofluorescence analysis of NIH-3T3 cells using LysRS/KARS Rabbit mAb (A8648) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LysRS/KARS Rabbit mAb (A8648)

Immunofluorescence analysis of U-2 OS cells using LysRS/KARS Rabbit mAb (A8648) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameLysRS/KARS Rabbit mAb
Catalog No.A8648
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1765
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 500-597 of human LysRS/KARS (Q15046).
SequenceTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV
Gene ID
Swiss Prot
SynonymsKRS; KARS; KARS2; LEPID; CMTRIB; DEAPLE; DFNB89; LysRS/KARS
Calculated MW68kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, C6, Mouse testis, Rat testis
Cellular locationCell membrane, Cytoplasm, Mitochondrion, Nucleus, Peripheral membrane protein, Secreted.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - LysRS/KARS Rabbit mAb (A8648)}

    Western blot - LysRS/KARS Rabbit mAb (A8648)

    Western blot analysis of various lysates using LysRS/KARS Rabbit mAb (A8648) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - LysRS/KARS Rabbit mAb (A8648)}

    Immunohistochemistry - LysRS/KARS Rabbit mAb (A8648)

    Immunohistochemistry analysis of paraffin-embedded Rat testis using LysRS/KARS Rabbit mAb (A8648) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - LysRS/KARS Rabbit mAb (A8648)}

    Immunohistochemistry - LysRS/KARS Rabbit mAb (A8648)

    Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using LysRS/KARS Rabbit mAb (A8648) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - LysRS/KARS Rabbit mAb (A8648)}

    Immunofluorescence - LysRS/KARS Rabbit mAb (A8648)

    Immunofluorescence analysis of C6 cells using LysRS/KARS Rabbit mAb (A8648) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - LysRS/KARS Rabbit mAb (A8648)}

    Immunofluorescence - LysRS/KARS Rabbit mAb (A8648)

    Immunofluorescence analysis of NIH-3T3 cells using LysRS/KARS Rabbit mAb (A8648) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - LysRS/KARS Rabbit mAb (A8648)}

    Immunofluorescence - LysRS/KARS Rabbit mAb (A8648)

    Immunofluorescence analysis of U-2 OS cells using LysRS/KARS Rabbit mAb (A8648) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)