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Lysozyme (LYZ) Rabbit pAb (A13511)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Lysozyme (LYZ) Rabbit pAb (A13511)

Western blot analysis of various lysates using Lysozyme (LYZ) Rabbit pAb (A13511) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Lysozyme (LYZ) Rabbit pAb (A13511)

Western blot analysis of various lysates using Lysozyme (LYZ) Rabbit pAb (A13511) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Lysozyme (LYZ) Rabbit pAb (A13511)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Lysozyme (LYZ) Rabbit pAb (A13511) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Lysozyme (LYZ) Rabbit pAb (A13511)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin using Lysozyme (LYZ) Rabbit pAb (A13511) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Lysozyme (LYZ) Rabbit pAb (A13511)

Immunofluorescence analysis of RAW264.7 cells using Lysozyme (LYZ) Rabbit mAb (A13511) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Lysozyme (LYZ) Rabbit pAb (A13511)

Immunofluorescence analysis of THP-1 cells using Lysozyme (LYZ) Rabbit mAb (A13511) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameLysozyme (LYZ) Rabbit pAb
Catalog No.A13511
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Lysozyme (Lysozyme (LYZ)) (NP_000230.1).
SequenceMKALIVLGLVLLSVTVQGKVFERCELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNACHLSCS
Gene ID
Swiss Prot
SynonymsLZM; LYZF1; Lysozyme (LYZ)
Calculated MW17kDa
Observed MW13kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHL-60, U-937, Mouse lung
Cellular locationSecreted.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Lysozyme (LYZ) Rabbit pAb (A13511)}

    Western blot - Lysozyme (LYZ) Rabbit pAb (A13511)

    Western blot analysis of various lysates using Lysozyme (LYZ) Rabbit pAb (A13511) at 1:3000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - Lysozyme (LYZ) Rabbit pAb (A13511)}

    Western blot - Lysozyme (LYZ) Rabbit pAb (A13511)

    Western blot analysis of various lysates using Lysozyme (LYZ) Rabbit pAb (A13511) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - Lysozyme (LYZ) Rabbit pAb (A13511)}

    Immunohistochemistry - Lysozyme (LYZ) Rabbit pAb (A13511)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Lysozyme (LYZ) Rabbit pAb (A13511) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Lysozyme (LYZ) Rabbit pAb (A13511)}

    Immunohistochemistry - Lysozyme (LYZ) Rabbit pAb (A13511)

    Immunohistochemistry analysis of paraffin-embedded Mouse intestin using Lysozyme (LYZ) Rabbit pAb (A13511) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Lysozyme (LYZ) Rabbit pAb (A13511)}

    Immunofluorescence - Lysozyme (LYZ) Rabbit pAb (A13511)

    Immunofluorescence analysis of RAW264.7 cells using Lysozyme (LYZ) Rabbit mAb (A13511) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Lysozyme (LYZ) Rabbit pAb (A13511)}

    Immunofluorescence - Lysozyme (LYZ) Rabbit pAb (A13511)

    Immunofluorescence analysis of THP-1 cells using Lysozyme (LYZ) Rabbit mAb (A13511) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A13511? Please let us know so that we can cite the reference in this datasheet.

    항체 (2)

    ELISA 키트 (1)

    Secondary Antibodies (26)