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MAFG Rabbit pAb (A16056)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - MAFG Rabbit pAb (A16056)

Western blot analysis of various lysates using MAFG Rabbit pAb (A16056) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameMAFG Rabbit pAb
Catalog No.A16056
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human MAFG (NP_002350.1).
SequenceMTTPNKGNKALKVKREPGENGTSLTDEELVTMSVRELNQHLRGLSKEEIVQLKQRRRTLKNRGYAASCRVKRVTQKEELEKQKAELQQEVEKLASENASMKLELDALRSKYEALQTFARTVARSPVAPARGPLAAGLGPLVPGKVAATSVITIVKSKTDARS
Gene ID
Swiss Prot
SynonymshMAF; MAFG
Calculated MW18kDa
Observed MW17kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse kidney
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - MAFG Rabbit pAb (A16056)}

Western blot - MAFG Rabbit pAb (A16056)

Western blot analysis of various lysates using MAFG Rabbit pAb (A16056) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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