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MAP3K4 Rabbit mAb (A20874)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MAP3K4 Rabbit mAb (A20874)

Western blot analysis of lysates from Rat testis, using MAP3K4 Rabbit mAb (A20874) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - MAP3K4 Rabbit mAb (A20874)

Immunohistochemistry analysis of MAP3K4 in paraffin-embedded rat heart tissue using MAP3K4 Rabbit mAb (A20874) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MAP3K4 Rabbit mAb (A20874)

Immunohistochemistry analysis of MAP3K4 in paraffin-embedded human liver cancer tissue using MAP3K4 Rabbit mAb (A20874) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MAP3K4 Rabbit mAb (A20874)

Immunohistochemistry analysis of MAP3K4 in paraffin-embedded human small intestine tissue using MAP3K4 Rabbit mAb (A20874) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MAP3K4 Rabbit mAb (A20874)

Immunohistochemistry analysis of MAP3K4 in paraffin-embedded mouse heart tissue using MAP3K4 Rabbit mAb (A20874) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameMAP3K4 Rabbit mAb
Catalog No.A20874
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2815
The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human MAP3K4 (Q9Y6R4).
SequenceQRNNVGRPASRSNLKEKMNAPNQPPHKDTGKTVENVEEYSYKQEKKIRAALRTTERDRKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPV
Gene ID
Swiss Prot
SynonymsMTK1; MEKK4; MEKK 4; MAPKKK4; PRO0412; MAP3K4
Calculated MW182kDa
Observed MW180kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRat testis
Cellular locationcytoplasm, perinuclear region of cytoplasm

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ABclonal:Western blot - MAP3K4 Rabbit mAb (A20874)}

Western blot - MAP3K4 Rabbit mAb (A20874)

Western blot analysis of lysates from Rat testis, using MAP3K4 Rabbit mAb (A20874) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - MAP3K4 Rabbit mAb (A20874)}

Immunohistochemistry - MAP3K4 Rabbit mAb (A20874)

Immunohistochemistry analysis of MAP3K4 in paraffin-embedded rat heart tissue using MAP3K4 Rabbit mAb (A20874) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MAP3K4 Rabbit mAb (A20874)}

Immunohistochemistry - MAP3K4 Rabbit mAb (A20874)

Immunohistochemistry analysis of MAP3K4 in paraffin-embedded human liver cancer tissue using MAP3K4 Rabbit mAb (A20874) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MAP3K4 Rabbit mAb (A20874)}

Immunohistochemistry - MAP3K4 Rabbit mAb (A20874)

Immunohistochemistry analysis of MAP3K4 in paraffin-embedded human small intestine tissue using MAP3K4 Rabbit mAb (A20874) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MAP3K4 Rabbit mAb (A20874)}

Immunohistochemistry - MAP3K4 Rabbit mAb (A20874)

Immunohistochemistry analysis of MAP3K4 in paraffin-embedded mouse heart tissue using MAP3K4 Rabbit mAb (A20874) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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