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MARS Rabbit pAb (A9938)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - MARS Rabbit pAb (A9938)

Western blot analysis of extracts of various cell lines, using MARS antibody (A9938) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameMARS Rabbit pAb
Catalog No.A9938
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MARS (NP_004981.2).
SequenceMRLFVSDGVPGCLPVLAAAGRARGRAEVLISTVGPEDCVVPFLTRPKVPVLQLDSGNYLFSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKKGEDVLGSVRRALTHIDHSLSRQNCPFLAGETESLADIVLWGALYPLLQDPAYLPEELSALHSWFQTLSTQEPCQRAAETVLKQQGVLALRPYLQKQPQPSPAEGRAVTNEPEEEELATLSEEEIAMAVTAWEKGLESLPPLRPQQNPVLPVAGERNVLITS
Gene ID
Swiss Prot
SynonymsMRS; ILLD; MARS; TTD9; CMT2U; ILFS2; METRS; MTRNS; SPG70
Calculated MW101kDa
Observed MW110kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSGC-7901, HepG2, HeLa, SW480, HT-1080
Cellular locationCytoplasm
Customer validation

WB(Homo sapiens)

Documents

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    ABclonal:Western blot - MARS Rabbit pAb (A9938)}

    Western blot - MARS Rabbit pAb (A9938)

    Western blot analysis of extracts of various cell lines, using MARS antibody (A9938) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A9938? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)