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MBTPS1 Rabbit PolymAb® (A27314PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - MBTPS1 Rabbit PolymAb® (A27314PM)

Western blot analysis of lysates from Mouse lung using MBTPS1 Rabbit PolymAb® (A27314PM) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Western blot - MBTPS1 Rabbit PolymAb® (A27314PM)

Western blot analysis of lysates from Rat lung using MBTPS1 Rabbit PolymAb® (A27314PM) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - MBTPS1 Rabbit PolymAb® (A27314PM)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using MBTPS1 Rabbit PolymAb® (A27314PM) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MBTPS1 Rabbit PolymAb® (A27314PM)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using MBTPS1 Rabbit PolymAb® (A27314PM) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product nameMBTPS1 Rabbit PolymAb®
Catalog No.A27314PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC72174_ARC72176
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the cis/medial-Golgi where a second autocatalytic event takes place and the catalytic activity is acquired. It encodes a type 1 membrane bound protease which is ubiquitously expressed and regulates cholesterol or lipid homeostasis via cleavage of substrates at non-basic residues. Mutations in this gene may be associated with lysosomal dysfunction.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 953-1052 of human MBTPS1 (NP_003782.1).
SequenceLSIDLDKVVLPNFRSNRPQVRPLSPGESGAWDIPGGIMPGRYNQEVGQTIPVFAFLGAMVVLAFFVVQINKAKSRPKRRKPRVKRPQLMQQVHPPKTPSV
Gene ID
Swiss Prot
SynonymsS1P; PCSK8; SEDKF; SKI-1
Calculated MW118kDa
Observed MW100kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRat lung, Mouse lung
Cellular locationEndoplasmic reticulum membrane, Golgi apparatus membrane, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - MBTPS1 Rabbit PolymAb® (A27314PM)}

    Western blot - MBTPS1 Rabbit PolymAb® (A27314PM)

    Western blot analysis of lysates from Mouse lung using MBTPS1 Rabbit PolymAb® (A27314PM) at 1:1000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.
    ABclonal:Western blot - MBTPS1 Rabbit PolymAb® (A27314PM)}

    Western blot - MBTPS1 Rabbit PolymAb® (A27314PM)

    Western blot analysis of lysates from Rat lung using MBTPS1 Rabbit PolymAb® (A27314PM) at 1:1000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - MBTPS1 Rabbit PolymAb® (A27314PM)}

    Immunohistochemistry - MBTPS1 Rabbit PolymAb® (A27314PM)

    Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using MBTPS1 Rabbit PolymAb® (A27314PM) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - MBTPS1 Rabbit PolymAb® (A27314PM)}

    Immunohistochemistry - MBTPS1 Rabbit PolymAb® (A27314PM)

    Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using MBTPS1 Rabbit PolymAb® (A27314PM) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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