제품 > 항체 > 단일클론항체(mAb)

MC5 Receptor Rabbit mAb (A9331)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MC5 Receptor Rabbit mAb (A9331)

Western blot analysis of extracts of various cell lines, using (A9331) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product nameMC5 Receptor Rabbit mAb
Catalog No.A9331
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2739
This gene encodes a member of the seven-pass transmembrane G protein-coupled melanocortin receptor protein family that stimulate cAMP signal transduction. The encoded protein is a receptor for melanocyte-stimulating hormone and adrenocorticotropic hormone and is suggested to play a role in sebum generation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human MC5 Receptor (P33032).
SequenceMNSSFHLHFLDLNLNATEGNLSGPNVKNKSSPCEDMGIAVEVFLTLGVISLLENILVIGAIVKNKNLHSPMYFFVCSLAVADMLVSMSSAWETITIYLLN
Gene ID
Swiss Prot
SynonymsMC2
Calculated MW37kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesPC-12, DU145, Mouse skeletal muscle, Mouse brain, Mouse spleen, Rat lung, Rat spleen
Cellular locationCell membrane, Multi-pass membrane protein

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ABclonal:Western blot - MC5 Receptor Rabbit mAb (A9331)}

Western blot - MC5 Receptor Rabbit mAb (A9331)

Western blot analysis of extracts of various cell lines, using (A9331) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)