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MDC1 Rabbit pAb (A8358)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - MDC1 Rabbit pAb (A8358)

Western blot analysis of various lysates using MDC1 Rabbit pAb (A8358) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 5s.

ABclonal:Immunohistochemistry - MDC1 Rabbit pAb (A8358)

Immunohistochemistry analysis of paraffin-embedded Human spleen using MDC1 Rabbit pAb (A8358) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MDC1 Rabbit pAb (A8358)

Immunohistochemistry analysis of paraffin-embedded Human testis using MDC1 Rabbit pAb (A8358) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - MDC1 Rabbit pAb (A8358)

Immunofluorescence analysis of U-2 OS cells using MDC1 Rabbit pAb (A8358) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMDC1 Rabbit pAb
Catalog No.A8358
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1 (NP_055456.2).
SequenceMEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKR
Gene ID
Swiss Prot
SynonymsNFBD1; MDC1
Calculated MW227kDa
Observed MW270kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, LO2
Cellular locationChromosome, Nucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - MDC1 Rabbit pAb (A8358)}

Western blot - MDC1 Rabbit pAb (A8358)

Western blot analysis of various lysates using MDC1 Rabbit pAb (A8358) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 5s.
ABclonal:Immunohistochemistry - MDC1 Rabbit pAb (A8358)}

Immunohistochemistry - MDC1 Rabbit pAb (A8358)

Immunohistochemistry analysis of paraffin-embedded Human spleen using MDC1 Rabbit pAb (A8358) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MDC1 Rabbit pAb (A8358)}

Immunohistochemistry - MDC1 Rabbit pAb (A8358)

Immunohistochemistry analysis of paraffin-embedded Human testis using MDC1 Rabbit pAb (A8358) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - MDC1 Rabbit pAb (A8358)}

Immunofluorescence - MDC1 Rabbit pAb (A8358)

Immunofluorescence analysis of U-2 OS cells using MDC1 Rabbit pAb (A8358) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)