제품 > 항체 > 다중클론항체(pAb)

MED6 Rabbit pAb (A8181)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - MED6 Rabbit pAb (A8181)

Western blot analysis of extracts of various cell lines, using MED6 antibody (A8181) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Overview

Product nameMED6 Rabbit pAb
Catalog No.A8181
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables transcription coactivator activity. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of mediator complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 27-246 of human MED6 (NP_005457.2).
SequenceGSVLDYFSERSNPFYDRTCNNEVVKMQRLTLEHLNQMVGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQDKVRPKAKRKEEPSSIFQRQRVDALLLDLRQKFPPKFVQLKPGEKPVPVDQTKKEAEPIPETVKPEEKETTKNVQQTVSAKGPPEKRMRLQ
Gene ID
Swiss Prot
SynonymsARC33; NY-REN-28
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7, Jurkat, HeLa
Cellular locationNucleus

Documents

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    ABclonal:Western blot - MED6 Rabbit pAb (A8181)}

    Western blot - MED6 Rabbit pAb (A8181)

    Western blot analysis of extracts of various cell lines, using MED6 antibody (A8181) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)