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MEK3 Rabbit pAb (A7718)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MEK3 Rabbit pAb (A7718)

Western blot analysis of various lysates using MEK3 Rabbit pAb (A7718) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - MEK3 Rabbit pAb (A7718)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using MEK3 Rabbit pAb (A7718) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MEK3 Rabbit pAb (A7718)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MEK3 Rabbit pAb (A7718) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MEK3 Rabbit pAb (A7718)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MEK3 Rabbit pAb (A7718) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - MEK3 Rabbit pAb (A7718)

Immunofluorescence analysis of C6 cells using MEK3 Rabbit pAb (A7718) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MEK3 Rabbit pAb (A7718)

Immunofluorescence analysis of NIH/3T3 cells using MEK3 Rabbit pAb (A7718) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MEK3 Rabbit pAb (A7718)

Immunofluorescence analysis of U-2 OS cells using MEK3 Rabbit pAb (A7718) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMEK3 Rabbit pAb
Catalog No.A7718
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human MEK3 (NP_659731.1).
SequenceMESPASSQPASMPQSKGKSKRKKDLRISCMSKPPAPNPTPPRNLDSRTFITIGDRNFEVEADDLVTISEL
Gene ID
Swiss Prot
SynonymsMEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Calculated MW39kDa
Observed MW39kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse heart, Mouse skeletal muscle
Cellular locationcytosol, nucleoplasm
Customer validation

WB(Mus musculus, Oryza sativa)

IP(Oryza sativa)

Documents

Certificate of Compliance

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ABclonal:Western blot - MEK3 Rabbit pAb (A7718)}

Western blot - MEK3 Rabbit pAb (A7718)

Western blot analysis of various lysates using MEK3 Rabbit pAb (A7718) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - MEK3 Rabbit pAb (A7718)}

Immunohistochemistry - MEK3 Rabbit pAb (A7718)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using MEK3 Rabbit pAb (A7718) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MEK3 Rabbit pAb (A7718)}

Immunohistochemistry - MEK3 Rabbit pAb (A7718)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MEK3 Rabbit pAb (A7718) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MEK3 Rabbit pAb (A7718)}

Immunohistochemistry - MEK3 Rabbit pAb (A7718)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MEK3 Rabbit pAb (A7718) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - MEK3 Rabbit pAb (A7718)}

Immunofluorescence - MEK3 Rabbit pAb (A7718)

Immunofluorescence analysis of C6 cells using MEK3 Rabbit pAb (A7718) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MEK3 Rabbit pAb (A7718)}

Immunofluorescence - MEK3 Rabbit pAb (A7718)

Immunofluorescence analysis of NIH/3T3 cells using MEK3 Rabbit pAb (A7718) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MEK3 Rabbit pAb (A7718)}

Immunofluorescence - MEK3 Rabbit pAb (A7718)

Immunofluorescence analysis of U-2 OS cells using MEK3 Rabbit pAb (A7718) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A7718? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)