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METTL13 Rabbit pAb (A12582)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - METTL13 Rabbit pAb (A12582)

Western blot analysis of extracts of MCF7 cells, using METTL13 antibody (A12582) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunofluorescence - METTL13 Rabbit pAb (A12582)

Immunofluorescence analysis of A549 cells using METTL13 antibody (A12582). Blue: DAPI for nuclear staining.

Overview

Product nameMETTL13 Rabbit pAb
Catalog No.A12582
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable methyltransferase activity. Involved in negative regulation of cell cycle G1/S phase transition and negative regulation of transcription by RNA polymerase II. Predicted to be located in mitochondrion and nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 430-699 of human METTL13 (NP_057019.3).
SequenceLKDVSHKAQKKRKKDRKKQRPADAEDLPAAPGQSIDKSYLCCEHHKAMIAGLALLRNPELLLEIPLALLVVGLGGGSLPLFVHDHFPKSCIDAVEIDPSMLEVATQWFGFSQSDRMKVHIADGLDYIASLAGGGEARPCYDVIMFDVDSKDPTLGMSCPPPAFVEQSFLQKVKSILTPEGVFILNLVCRDLGLKDSVLAGLKAVFPLLYVRRIEGEVNEILFCQLHPEQKLATPELLETAQALERTLRKPGRGWDDTYVLSDMLKTVKIV
Gene ID
Swiss Prot
Synonymsfeat; DFNM1; CGI-01; DFNB26; DFNB26M; KIAA0859; EEF1AKNMT; 5630401D24Rik
Calculated MW79kDa
Observed MW85kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7
Cellular locationcytoplasm, mitochondrion, nucleus

Documents

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    ABclonal:Western blot - METTL13 Rabbit pAb (A12582)}

    Western blot - METTL13 Rabbit pAb (A12582)

    Western blot analysis of extracts of MCF7 cells, using METTL13 antibody (A12582) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - METTL13 Rabbit pAb (A12582)}

    Immunofluorescence - METTL13 Rabbit pAb (A12582)

    Immunofluorescence analysis of A549 cells using METTL13 antibody (A12582). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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