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METTL4 Rabbit pAb (A25600)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - METTL4 Rabbit pAb (A25600)

Immunohistochemistry analysis of METTL4 in paraffin-embedded Human liver cancer tissue using METTL4 Rabbit pAb (A25600) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - METTL4 Rabbit pAb (A25600)

Immunohistochemistry analysis of METTL4 in paraffin-embedded Human lung tissue using METTL4 Rabbit pAb (A25600) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - METTL4 Rabbit pAb (A25600)

Immunohistochemistry analysis of METTL4 in paraffin-embedded Mouse heart tissue using METTL4 Rabbit pAb (A25600) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - METTL4 Rabbit pAb (A25600)

Immunohistochemistry analysis of METTL4 in paraffin-embedded Rat heart tissue using METTL4 Rabbit pAb (A25600) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - METTL4 Rabbit pAb (A25600)

Immunofluorescence analysis of Hep G2 cells using METTL4 Rabbit pAb (A25600) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMETTL4 Rabbit pAb
Catalog No.A25600
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables RNA methyltransferase activity and site-specific DNA-methyltransferase (adenine-specific) activity. Involved in nucleic acid metabolic process; regulation of RNA metabolic process; and regulation of mitochondrial DNA replication. Located in cytosol; mitochondrial matrix; and nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 280-472 of human METTL4 (NP_073751.3).
SequenceTFDVIVIDPPWQNKSVKRSNRYSYLSPLQIQQIPIPKLAAPNCLLVTWVTNRQKHLRFIKEELYPSWSVEVVAEWHWVKITNSGEFVFPLDSPHKKPYEGLILGRVQEKTALPLRNADVNVLPIPDHKLIVSVPCTLHSHKPPLAEVLKDYIKPDGEYLELFARNLQPGWTSWGNEVLKFQHVDYFIAVESGS
Gene ID
Swiss Prot
SynonymsHsT661
Calculated MW54kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IHC 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationImmunohistochemistry    Immunofluorescence    
Positive samples
Cellular locationNucleus, Cytoplasm, cytosol, mitochondrial matrix, nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunohistochemistry - METTL4 Rabbit pAb (A25600)}

    Immunohistochemistry - METTL4 Rabbit pAb (A25600)

    Immunohistochemistry analysis of METTL4 in paraffin-embedded Human liver cancer tissue using METTL4 Rabbit pAb (A25600) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - METTL4 Rabbit pAb (A25600)}

    Immunohistochemistry - METTL4 Rabbit pAb (A25600)

    Immunohistochemistry analysis of METTL4 in paraffin-embedded Human lung tissue using METTL4 Rabbit pAb (A25600) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - METTL4 Rabbit pAb (A25600)}

    Immunohistochemistry - METTL4 Rabbit pAb (A25600)

    Immunohistochemistry analysis of METTL4 in paraffin-embedded Mouse heart tissue using METTL4 Rabbit pAb (A25600) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - METTL4 Rabbit pAb (A25600)}

    Immunohistochemistry - METTL4 Rabbit pAb (A25600)

    Immunohistochemistry analysis of METTL4 in paraffin-embedded Rat heart tissue using METTL4 Rabbit pAb (A25600) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - METTL4 Rabbit pAb (A25600)}

    Immunofluorescence - METTL4 Rabbit pAb (A25600)

    Immunofluorescence analysis of Hep G2 cells using METTL4 Rabbit pAb (A25600) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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