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MGMT Rabbit pAb (A0693)

Reviews (3)Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - MGMT Rabbit pAb (A0693)

Western blot analysis of extracts of various cell lines, using MGMT antibody (A0693) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Review
ABclonal: review for MGMT Rabbit pAb(A0693)
Review
ABclonal: review for MGMT Rabbit pAb(A0693)
Review
ABclonal: review for MGMT Rabbit pAb(A0693)

Overview

Product nameMGMT Rabbit pAb
Catalog No.A0693
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human MGMT (NP_002403.2).
SequenceMLGQPAPLERFASRRPQVLAVRTVCDLVLGKMDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHRL
Gene ID
Swiss Prot
SynonymsMGMT
Calculated MW22kDa
Observed MW22kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat, Mouse liver, Mouse spleen
Cellular locationNucleus
Customer validation

(Human glioma tissue, HepG2)

WB(Homo sapiens)

Documents

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ABclonal:Western blot - MGMT Rabbit pAb (A0693)}

Western blot - MGMT Rabbit pAb (A0693)

Western blot analysis of extracts of various cell lines, using MGMT antibody (A0693) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)