제품 > 항체 > 다중클론항체(pAb)

MIOX Rabbit pAb (A14409)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - MIOX Rabbit pAb (A14409)

Western blot analysis of various lysates using MIOX Rabbit pAb (A14409) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunofluorescence - MIOX Rabbit pAb (A14409)

Immunofluorescence analysis of paraffin-embedded Rat kidney using MIOX Rabbit pAb (A14409) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MIOX Rabbit pAb (A14409)

Immunofluorescence analysis of paraffin-embedded Mouse kidney using MIOX Rabbit pAb (A14409) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMIOX Rabbit pAb
Catalog No.A14409
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables ferric iron binding activity and inositol oxygenase activity. Involved in inositol catabolic process. Predicted to be located in cytoplasm and inclusion body.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human MIOX (NP_060054.4).
SequenceMKVTVGPDPSLVYRPDVDPEVAKDKASFRNYTSGPLLDRVFTTYKLMHTHQTVDFVRSKHAQFGGFSYKKMTVMEAVDLLDGLVDESDPDVDFPNSFHAFQTAEGIRKAHPDKDWFHLVGLLHDLGKVLALFGEPQWAVVGDTFPVGCRPQASVVFCDSTFQDNPDLQDPRYSTELGMYQPHCGLDRVLMSWGHDEYMYQVMKFNKFSLPPEAFYMIRFHSFYPWHTGRDYQQLCSQQDLAMLPWVREFNKFDLYTKCPDLPDVDKLRPYYQGLIDKYCPGILSW
Gene ID
Swiss Prot
SynonymsALDRL6; MIOX
Calculated MW33kDa
Observed MW33kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse kidney, Rat kidney
Cellular locationCytoplasm

Documents

Certificate of Compliance

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    ABclonal:Western blot - MIOX Rabbit pAb (A14409)}

    Western blot - MIOX Rabbit pAb (A14409)

    Western blot analysis of various lysates using MIOX Rabbit pAb (A14409) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 15s.
    ABclonal:Immunofluorescence - MIOX Rabbit pAb (A14409)}

    Immunofluorescence - MIOX Rabbit pAb (A14409)

    Immunofluorescence analysis of paraffin-embedded Rat kidney using MIOX Rabbit pAb (A14409) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - MIOX Rabbit pAb (A14409)}

    Immunofluorescence - MIOX Rabbit pAb (A14409)

    Immunofluorescence analysis of paraffin-embedded Mouse kidney using MIOX Rabbit pAb (A14409) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.