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MITF Rabbit mAb (A22091)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - MITF Rabbit mAb (A22091)

Western blot analysis of lysates from Hela cells, using MITF Rabbit mAb (A22091) at1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - MITF Rabbit mAb (A22091)

Western blot analysis of lysates from Rat heart, using MITF Rabbit mAb (A22091) at1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - MITF Rabbit mAb (A22091)

Immunofluorescence analysis of HeLa cells using MITF Rabbit mAb (A22091) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMITF Rabbit mAb
Catalog No.A22091
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55801
The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 402-526 of human MITF (NP_001341533.1).
SequenceHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQDLLQHHADLTCTTTLDLTDGTITFNNNLGTGTEANQAYSVPTKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSMSMEETEHTC
Gene ID
Swiss Prot
SynonymsMI; WS2; CMM8; WS2A; COMMAD; MITF-A; bHLHe32; MITF
Calculated MW59kDa
Observed MW50-75kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:20000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, Rat heart
Cellular locationNucleus, Cytoplasm

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ABclonal:Western blot - MITF Rabbit mAb (A22091)}

Western blot - MITF Rabbit mAb (A22091)

Western blot analysis of lysates from Hela cells, using MITF Rabbit mAb (A22091) at1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - MITF Rabbit mAb (A22091)}

Western blot - MITF Rabbit mAb (A22091)

Western blot analysis of lysates from Rat heart, using MITF Rabbit mAb (A22091) at1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunofluorescence - MITF Rabbit mAb (A22091)}

Immunofluorescence - MITF Rabbit mAb (A22091)

Immunofluorescence analysis of HeLa cells using MITF Rabbit mAb (A22091) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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