제품 > 항체 > 다중클론항체(pAb)

MITF Rabbit pAb (A1255)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of extracts of various cell lines, using MITF antibody (A1255) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	MITF Rabbit pAb
Catalog No.	A1255
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MITF (NP_937801.1).
Sequence	MLEMLEYNHYQVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQVLSLPCPNQPGDHVMPPVPGSSAPNSPMAMLTLNSNCEKEGFYKFEEQNRAESECPGMNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQGLPPPGLTISNSCPANLPNIKRELTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRAKELENRQKKLEHANRHLLLRIQ
Gene ID
Swiss Prot
Synonyms	MI; WS2; CMM8; WS2A; COMMAD; MITF-A; bHLHe32
Calculated MW	59kDa
Observed MW	50-70kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Jurkat, K-562, Mouse kidney
Cellular location	Nucleus
Customer validation	WB(Mactra, Meretrix meretrix, Meretrix petechialis, Homo sapiens, Danio rerio, Other, Crassostrea gigas)

Documents

Certificate of Compliance

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Lot number

Research Area

MITF Rabbit pAb images

Western blot - MITF Rabbit pAb (A1255)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for MITF Rabbit pAb (A1255)

Product:

MITF Rabbit pAb

Journal:

Fish & shellfish Immunology

Application:

IF:

4.1

Species:

Mactra

PMID:

28734968

Title:

Identification of an MITF gene and its polymorphisms associated with the Vibrio resistance trait in the clam Meretrix petechialis

References for MITF Rabbit pAb (A1255)

Product:

MITF Rabbit pAb

Journal:

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

Application:

IF:

1.9

Species:

Mactra

PMID:

30031885

Title:

Identification of a gene encoding microphthalmia-associated transcription factor and its association with shell color in the clam Meretrix petechialis

References for MITF Rabbit pAb (A1255)

Product:

MITF Rabbit pAb

Journal:

Frontiers in Immunology

Application:

IF:

5.7

Species:

Meretrix meretrix

PMID:

31333673