제품 > 항체 > 다중클론항체(pAb)

MKP1 Rabbit pAb (A23261)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MKP1 Rabbit pAb (A23261)

Western blot analysis of various lysates, using MKP1 Rabbit pAb (A23261) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 2s.

ABclonal:Western blot - MKP1 Rabbit pAb (A23261)

Western blot analysis of various lysates, using MKP1 Rabbit pAb (A23261) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 2s.

Overview

Product nameMKP1 Rabbit pAb
Catalog No.A23261
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables mitogen-activated protein kinase binding activity and phosphoprotein phosphatase activity. Involved in negative regulation of MAPK cascade; negative regulation of cell population proliferation; and protein dephosphorylation. Predicted to be active in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; limb; and sensory organ. Human ortholog(s) of this gene implicated in breast cancer. Orthologous to human DUSP1 (dual specificity phosphatase 1).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-172 of mouse MKP1. (NP_038670.1).
SequenceMVMEVGILDAGGLRALLREGAAQCLLLDCRSFFAFNAGHIAGSVNVRFSTIVRRRAKGAMGLEHIVPNAELRGRLLAGAYHAVVLLDERSASLDGAKRDGTLALAAGALCREARSTQVFFLQGGYEAFSASCPELCSKQSTPTGLSLPLSTSVPDSAESGCSSCSTPLYDQG
Gene ID
Swiss Prot
Synonymserp; MKP1; mkp-1; 3CH134; Ptpn16
Calculated MW39kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, HL-60, Mouse lung, Rat lung, Rat testis
Cellular location

Documents

Certificate of Compliance

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    ABclonal:Western blot - MKP1 Rabbit pAb (A23261)}

    Western blot - MKP1 Rabbit pAb (A23261)

    Western blot analysis of various lysates, using MKP1 Rabbit pAb (A23261) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 2s.
    ABclonal:Western blot - MKP1 Rabbit pAb (A23261)}

    Western blot - MKP1 Rabbit pAb (A23261)

    Western blot analysis of various lysates, using MKP1 Rabbit pAb (A23261) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 2s.

    * For research use only. Not for therapeutic or diagnostic purposes.