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MKP1 Rabbit pAb (A24667)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - MKP1 Rabbit pAb (A24667)

Western blot analysis of various lysates, using MKP1 Rabbit pAb (A24667) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC):THP-1
Exposure time: 3s.

Overview

Product nameMKP1 Rabbit pAb
Catalog No.A24667
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a phosphatase with dual specificity for tyrosine and threonine. The encoded protein can dephosphorylate MAP kinase MAPK1/ERK2, which results in its involvement in several cellular processes. This protein appears to play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. Finally, the encoded protein can make some solid tumors resistant to both chemotherapy and radiotherapy, making it a target for cancer therapy.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 267-367 of human MKP1 (NP_004408.1).
SequenceTICLAYLMRTNRVKLDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHSTNSALSYLQSPITTSPSC
Gene ID
Swiss Prot
SynonymsHVH1; MKP1; CL100; MKP-1; PTPN10
Calculated MW39kDa
Observed MW40kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa
Cellular locationNucleus.

Documents

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ABclonal:Western blot - MKP1 Rabbit pAb (A24667)}

Western blot - MKP1 Rabbit pAb (A24667)

Western blot analysis of various lysates, using MKP1 Rabbit pAb (A24667) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC):THP-1
Exposure time: 3s.

* For research use only. Not for therapeutic or diagnostic purposes.

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