제품 > 항체 > 단일클론항체(mAb)

MLKL Rabbit mAb (A19685)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates using MLKL Rabbit mAb (A19685) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name	MLKL Rabbit mAb
Catalog No.	A19685
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0165

Background

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 373-471 of human MLKL (Q8NB16).
Sequence	STAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK
Gene ID
Swiss Prot
Synonyms	hMLKL; MLKL
Calculated MW	54kDa
Observed MW	54kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Jurkat, HeLa
Cellular location	Cell membrane, Cytoplasm.
Customer validation	WB(Gallus gallus, Mus musculus, Rattus norvegicus, Homo sapiens, Rattus norvegicus, Ctenopharyngodon idella, Mus musculus) IHC(Mus musculus) IHC(Mus musculus) IF(Mus musculus) ELISA(Mus musculus) RNA-Seq(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

MLKL Rabbit mAb images

Western blot - MLKL Rabbit mAb (A19685)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

J Hazard Mater

Application:

IF:

12.2

Species:

Gallus gallus

PMID:

33307448

Title:

Regulation of H 2 S-induced necroptosis and inflammation in broiler bursa of Fabricius by the miR-15b-5p/TGFBR3 axis and the involvement of oxidative stress in this process

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.6

Species:

Mus musculus

PMID:

36238555

Title:

Selumetinib - a potential small molecule inhibitor for osteoarthritis treatment - PMC

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

iScience

Application:

IHC

IF:

5.8

Species:

Mus musculus

PMID:

36465134

Title:

Role of pericytes in the development of cerebral cavernous malformations - PMC

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

Application:

IF:

3.9

Species:

Gallus gallus

PMID:

36375805

Title:

Roles of selenoprotein K in oxidative stress and endoplasmic reticulum stress under selenium deficiency in chicken liver

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

Cell proliferation

Application:

IF:

8.5

Species:

Mus musculus

PMID:

36810909

Title:

Dietary nitrate improves jaw bone remodelling in zoledronate‐treated mice

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

Neurochemical research

Application:

IF:

3.7

Species:

Rattus norvegicus

PMID:

36729311

Title:

taVNS Alleviates Sevoflurane-Induced Cognitive Dysfunction in Aged Rats Via Activating Basal Forebrain Cholinergic Neurons

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

Development (Cambridge, England)

Application:

IF:

3.7

Species:

Mus musculus

PMID:

36975381

Title:

S-adenosyl-L-methionine supplementation alleviates the destructed intestinal epithelium and inflammatory infiltration caused by Mat2a deficiency

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

European journal of medicinal chemistry

Application:

IF:

Species:

Homo sapiens

PMID:

37494773

Title:

Identification of Pyrido [3, 4-D] Pyrimidine Derivatives as Ripk3-Mediated Necroptosis Inhibitors

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

European journal of pharmacology

Application:

IF:

4.2

Species:

Mus musculus, Rattus norvegicus

PMID:

37572943

Title:

Kinsenoside mitigates myocardial ischemia/reperfusion-induced ferroptosis via activation of the Akt/Nrf2/HO-1 pathway

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

Fish & shellfish immunology

Application:

IF:

4.1

Species:

Ctenopharyngodon idella

PMID:

37573970

Title:

Evodiamine alleviates DEHP-induced hepatocyte pyroptosis, necroptosis and immunosuppression in grass carp through ROS-regulated TLR4/MyD88/NF-B pathway

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

International immunopharmacology

Application:

WB, IHC, IF, ELISA

IF:

4.8

Species:

Mus musculus

PMID:

35952515

Title:

AZ-628 delays osteoarthritis progression via inhibiting the TNF--induced chondrocyte necroptosis and regulating osteoclast formation

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

The Journal of pharmacy and pharmacology

Application:

IF:

2.8

Species:

Rattus norvegicus

PMID:

36856818

Title:

Limonin delays the progression of intervertebral disc degeneration in vivo and in vitro: the key role of the MAPK/NF-B and necroptosis pathways

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

Clinical and translational medicine

Application:

IF:

10.6

Species:

Homo sapiens, Mus musculus

PMID:

37475188

Title:

RIPK3-MLKL necroptotic signalling amplifies STING pathway and exacerbates lethal sepsis - PMC

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

Cell death and differentiation

Application:

RNA-Seq

IF:

13.7

Species:

Mus musculus

PMID:

38783090

Title:

MLKL promotes hepatocarcinogenesis through inhibition of AMPK-mediated autophagy

References for MLKL Rabbit mAb (A19685)

Product:

MLKL Rabbit mAb

Journal:

Theranostics

Application:

IF:

12.4

Species:

Mus musculus

PMID:

39346531

Title:

Selenoprotein S maintains intestinal homeostasis in ulcerative colitis by inhibiting necroptosis of colonic epithelial cells through modulation of macrophage polarization - PMC