제품 > 항체 > 다중클론항체(pAb)

MLN Rabbit pAb (A6388)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - MLN Rabbit pAb (A6388)

Western blot analysis of various lysates using MLN Rabbit pAb (A6388) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - MLN Rabbit pAb (A6388)

Immunohistochemistry analysis of paraffin-embedded Human stomach using MLN Rabbit pAb (A6388) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MLN Rabbit pAb (A6388)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using MLN Rabbit pAb (A6388) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MLN Rabbit pAb (A6388)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using MLN Rabbit pAb (A6388) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameMLN Rabbit pAb
Catalog No.A6388
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a small peptide hormone that is secreted by cells of the small intestine to regulate gastrointestinal contractions and motility. Proteolytic processing of the secreted protein produces the mature peptide and a byproduct referred to as motilin-associated peptide (MAP). Three transcript variants encoding different preproprotein isoforms but the same mature peptide have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 26-115 of human MLN (NP_002409.1).
SequenceFVPIFTYGELQRMQEKERNKGQKKSLSVWQRSGEEGPVDPAEPIREEENEMIKLTAPLEIGMRMNSRQLEKYPATLEGLLSEMLPQHAAK
Gene ID
Swiss Prot
SynonymsMLN; motilin
Calculated MW13kDa
Observed MW13kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMCF7, SGC-7901, HT-29, Mouse stomach, Mouse intestine
Cellular locationSecreted

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - MLN Rabbit pAb (A6388)}

Western blot - MLN Rabbit pAb (A6388)

Western blot analysis of various lysates using MLN Rabbit pAb (A6388) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - MLN Rabbit pAb (A6388)}

Immunohistochemistry - MLN Rabbit pAb (A6388)

Immunohistochemistry analysis of paraffin-embedded Human stomach using MLN Rabbit pAb (A6388) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MLN Rabbit pAb (A6388)}

Immunohistochemistry - MLN Rabbit pAb (A6388)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using MLN Rabbit pAb (A6388) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MLN Rabbit pAb (A6388)}

Immunohistochemistry - MLN Rabbit pAb (A6388)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using MLN Rabbit pAb (A6388) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)