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MMP-1 Rabbit pAb (A24436)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Immunofluorescence - MMP-1 Rabbit pAb (A24436)

Immunofluorescence analysis of paraffin-embedded mouse testis using MMP-1 Rabbit pAb (A24436) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - MMP-1 Rabbit pAb (A24436)

Immunofluorescence analysis of paraffin-embedded rat testis using MMP-1 Rabbit pAb (A24436) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

Overview

Product nameMMP-1 Rabbit pAb
Catalog No.A24436
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 369-469 of human MMP1 (NP_002412.1).
SequenceFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN
Gene ID
Swiss Prot
SynonymsCLG; CLGN; MMP-1 (H440)
Calculated MW54kDa
Observed MWRefer to figures
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    
Positive samples
Cellular locationSecreted, extracellular matrix, extracellular space
Customer validation

WB(Homo sapiens)

Documents

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ABclonal:Immunofluorescence - MMP-1 Rabbit pAb (A24436)}

Immunofluorescence - MMP-1 Rabbit pAb (A24436)

Immunofluorescence analysis of paraffin-embedded mouse testis using MMP-1 Rabbit pAb (A24436) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - MMP-1 Rabbit pAb (A24436)}

Immunofluorescence - MMP-1 Rabbit pAb (A24436)

Immunofluorescence analysis of paraffin-embedded rat testis using MMP-1 Rabbit pAb (A24436) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)