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MMP25 Rabbit pAb (A3032)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MMP25 Rabbit pAb (A3032)

Western blot analysis of extracts of various cell lines, using MMP25 antibody (A3032) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - MMP25 Rabbit pAb (A3032)

Immunofluorescence analysis of C6 cells using MMP25 antibody (A3032) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MMP25 Rabbit pAb (A3032)

Immunofluorescence analysis of HeLa cells using MMP25 antibody (A3032) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MMP25 Rabbit pAb (A3032)

Immunofluorescence analysis of L929 cells using MMP25 antibody (A3032) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameMMP25 Rabbit pAb
Catalog No.A3032
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 350-540 of human MMP25 (NP_071913.1).
SequenceSPRPARLHRFWEGLPAQVRVVQAAYARHRDGRILLFSGPQFWVFQDRQLEGGARPLTELGLPPGEEVDAVFSWPQNGKTYLVRGRQYWRYDEAAARPDPGYPRDLSLWEGAPPSPDDVTVSNAGDTYFFKGAHYWRFPKNSIKTEPDAPQPMGPNWLDCPAPSSGPRAPRPPKATPVSETCDCQCELNQAA
Gene ID
Swiss Prot
SynonymsMMP20; MMPL1; MMP-25; MMP20A; MT6MMP; MTMMP6; MT-MMP6; MT6-MMP; MT-MMP 6
Calculated MW63kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesK-562, BT-474
Cellular locationCell membrane, Extracellular side, GPI-anchor, Lipid-anchor, Secreted, extracellular matrix, extracellular space

Documents

Certificate of Compliance

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ABclonal:Western blot - MMP25 Rabbit pAb (A3032)}

Western blot - MMP25 Rabbit pAb (A3032)

Western blot analysis of extracts of various cell lines, using MMP25 antibody (A3032) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - MMP25 Rabbit pAb (A3032)}

Immunofluorescence - MMP25 Rabbit pAb (A3032)

Immunofluorescence analysis of C6 cells using MMP25 antibody (A3032) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MMP25 Rabbit pAb (A3032)}

Immunofluorescence - MMP25 Rabbit pAb (A3032)

Immunofluorescence analysis of HeLa cells using MMP25 antibody (A3032) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MMP25 Rabbit pAb (A3032)}

Immunofluorescence - MMP25 Rabbit pAb (A3032)

Immunofluorescence analysis of L929 cells using MMP25 antibody (A3032) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)