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MMP9 Rabbit mAb (A25299)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NR8383 cells using MMP9 Rabbit mAb (A25299) at 1:1000 dilution. NR8383 cells were treated by LPS (100 ng/mL) for 4 hours and Brefeldin A (1μg/mL) for 3 hours of stimulation.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from RAW 264.7 cells using MMP9 Rabbit mAb (A25299) at 1:1000 dilution. Raw264.7 cells were treated by LPS (100 ng/mL) for 6 hours and Brefeldin A (300 ng/mL) for 3 hours of stimulation.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Western blot analysis of lysates from U-2 OS cells using MMP9 Rabbit mAb (A25299) at 1:1000 dilution incubated overnight at 4℃. U-2 OS cells were treated by PMA (200 nM) at 37℃ for 48h after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 10 s.

ABclonal:Immunohistochemistry - MMP9 Rabbit mAb (A25299)

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using MMP9 Rabbit mAb (A25299) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using MMP9 Rabbit mAb (A25299) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using MMP9 Rabbit mAb (A25299) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using MMP9 Rabbit mAb (A25299) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - MMP9 Rabbit mAb (A25299)

Confocal imaging of paraffin-embedded Human spleen tissue using MMP9 Rabbit mAb (A25299, dilution 1:50) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Confocal imaging of paraffin-embedded Human tonsil tissue using MMP9 Rabbit mAb (A25299, dilution 1:50) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Confocal imaging of paraffin-embedded Mouse spleen tissue using MMP9 Rabbit mAb (A25299, dilution 1:50) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product name	MMP9 Rabbit mAb
Catalog No.	A25299
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC3233

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Immunogen information

Immunogen	Synthetic peptide
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	MMP9; CLG4B; GELB; MANDP2; MMP-9; matrix metallopeptidase 9
Calculated MW	78kDa
Observed MW	84-92kDa/96kDa/84kDa/92kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:1000 - 1:5000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	NR8383 treated by LPS and BFA, RAW 264.7 treated by LPS and BFA, U-2 OS treated by PMA
Cellular location	Extracellular exosome, extracellular region, extracellular space.
Customer validation	WB(Gallus gallus, Mus musculus)