제품 > 항체 > 다중클론항체(pAb)

MMRN1 Rabbit pAb (A6658)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates, using MMRN1 Rabbit pAb (A6658) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC):U-251 MG
Exposure time: 180s.

ABclonal:Immunofluorescence - MMRN1 Rabbit pAb (A6658)

Immunofluorescence analysis of U2OS cells using MMRN1 antibody (A6658). Blue: DAPI for nuclear staining.

Overview

Product name	MMRN1 Rabbit pAb
Catalog No.	A6658
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Multimerin is a massive, soluble protein found in platelets and in the endothelium of blood vessels. It is comprised of subunits linked by interchain disulfide bonds to form large, variably sized homomultimers. Multimerin is a factor V/Va-binding protein and may function as a carrier protein for platelet factor V. It may also have functions as an extracellular matrix or adhesive protein. Recently, patients with an unusual autosomal-dominant bleeding disorder (factor V Quebec) were found to have a deficiency of platelet multimerin.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1083-1228 of human MMRN1 (NP_031377.2).
Sequence	ENALAPDFSKGSYRYAPMVAFFASHTYGMTIPGPILFNNLDVNYGASYTPRTGKFRIPYLGVYVFKYTIESFSAHISGFLVVDGIDKLAFESENINSEIHCDRVLTGDALLELNYGQEVWLRLAKGTIPAKFPPVTTFSGYLLYRT
Gene ID
Swiss Prot
Synonyms	ECM; MMRN; GPIa*; EMILIN4
Calculated MW	138kDa
Observed MW	180kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HepG2, U-251MG, Mouse lung, Mouse spleen
Cellular location	Secreted