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MOK Rabbit pAb (A1786)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MOK Rabbit pAb (A1786)

Western blot analysis of lysates from mouse liver, using MOK Rabbit pAb (A1786) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - MOK Rabbit pAb (A1786)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MOK Rabbit pAb (A1786) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - MOK Rabbit pAb (A1786)

Immunohistochemistry analysis of paraffin-embedded Rat heart using MOK Rabbit pAb (A1786) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - MOK Rabbit pAb (A1786)

Immunohistochemistry analysis of paraffin-embedded Human liver using MOK Rabbit pAb (A1786) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameMOK Rabbit pAb
Catalog No.A1786
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the MAP kinase superfamily. The gene was found to be regulated by caudal type transcription factor 2 (Cdx2) protein. The encoded protein, which is localized to epithelial cells in the intestinal crypt, may play a role in growth arrest and differentiation of cells of upper crypt and lower villus regions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MOK (NP_055041.1).
SequenceMKNYKAIGKIGEGTFSEVMKMQSLRDGNYYACKQMKQRFESIEQVNNLREIQALRRLNPHPNILMLHEVVFDRKSGSLALICELMDMNIYELIRGRRYPLSEKKIMHYMYQLCKSLDHIHRNGIFHRDVKPENILIKQDVLKLGDFGSCRSVYSKQPYTEYISTRWYRAPECLLTDGFYTYKMDLWSAGCVFYEIASLQPLFPGVNELDQISKIHDVIGTPAQKILTKFK
Gene ID
Swiss Prot
SynonymsRAGE; RAGE1; STK30; RAGE-1; MOK
Calculated MW48kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse liver
Cellular locationCytoplasm.

Documents

Certificate of Compliance

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ABclonal:Western blot - MOK Rabbit pAb (A1786)}

Western blot - MOK Rabbit pAb (A1786)

Western blot analysis of lysates from mouse liver, using MOK Rabbit pAb (A1786) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - MOK Rabbit pAb (A1786)}

Immunohistochemistry - MOK Rabbit pAb (A1786)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MOK Rabbit pAb (A1786) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - MOK Rabbit pAb (A1786)}

Immunohistochemistry - MOK Rabbit pAb (A1786)

Immunohistochemistry analysis of paraffin-embedded Rat heart using MOK Rabbit pAb (A1786) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - MOK Rabbit pAb (A1786)}

Immunohistochemistry - MOK Rabbit pAb (A1786)

Immunohistochemistry analysis of paraffin-embedded Human liver using MOK Rabbit pAb (A1786) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)