제품 > 항체 > 다중클론항체(pAb)

MR1 Rabbit pAb (A8051)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MR1 Rabbit pAb (A8051)

Western blot analysis of various lysates using MR1 Rabbit pAb (A8051) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - MR1 Rabbit pAb (A8051)

Immunofluorescence analysis of NIH/3T3 cells using MR1 Rabbit pAb (A8051) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MR1 Rabbit pAb (A8051)

Immunofluorescence analysis of PC-12 cells using MR1 Rabbit pAb (A8051) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMR1 Rabbit pAb
Catalog No.A8051
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
MAIT (mucosal-associated invariant T-cells) lymphocytes represent a small population of T-cells primarily found in the gut. The protein encoded by this gene is an antigen-presenting molecule that presents metabolites of microbial vitamin B to MAITs. This presentation may activate the MAITs to regulate the amounts of specific types of bacteria in the gut. Several transcript variants encoding different isoforms have been found for this gene, and a pseudogene of it has been detected about 36 kbp upstream on the same chromosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 23-291 of human MR1 (NP_001522.1).
SequenceRTHSLRYFRLGVSDPIHGVPEFISVGYVDSHPITTYDSVTRQKEPRAPWMAENLAPDHWERYTQLLRGWQQMFKVELKRLQRHYNHSGSHTYQRMIGCELLEDGSTTGFLQYAYDGQDFLIFNKDTLSWLAVDNVAHTIKQAWEANQHELLYQKNWLEEECIAWLKRFLEYGKDTLQRTEPPLVRVNRKETFPGVTALFCKAHGFYPPEIYMTWMKNGEEIVQEIDYGDILPSGDGTYQAWASIELDPQSSNLYSCHVEHCGVHMVLQV
Gene ID
Swiss Prot
SynonymsHLALS; MR1
Calculated MW39kDa
Observed MW39kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, Mouse lung, Mouse spleen, Rat brain
Cellular locationCell membrane, Endoplasmic reticulum, Endoplasmic reticulum membrane, Extracellular side, Secreted, Single-pass membrane protein, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - MR1 Rabbit pAb (A8051)}

    Western blot - MR1 Rabbit pAb (A8051)

    Western blot analysis of various lysates using MR1 Rabbit pAb (A8051) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - MR1 Rabbit pAb (A8051)}

    Immunofluorescence - MR1 Rabbit pAb (A8051)

    Immunofluorescence analysis of NIH/3T3 cells using MR1 Rabbit pAb (A8051) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - MR1 Rabbit pAb (A8051)}

    Immunofluorescence - MR1 Rabbit pAb (A8051)

    Immunofluorescence analysis of PC-12 cells using MR1 Rabbit pAb (A8051) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)