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MRPS15 Rabbit mAb (A9388)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - MRPS15 Rabbit mAb (A9388)

Western blot analysis of various lysates, using MRPS15 Rabbit mAb (A9388) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.

ABclonal:Immunohistochemistry - MRPS15 Rabbit mAb (A9388)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MRPS15 Rabbit mAb (A9388) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MRPS15 Rabbit mAb (A9388)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using MRPS15 Rabbit mAb (A9388) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - MRPS15 Rabbit mAb (A9388)

Immunofluorescence analysis of U2OS cells using MRPS15 Rabbit mAb (A9388) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMRPS15 Rabbit mAb
Catalog No.A9388
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2751
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 158-257 of human MRPS15 (P82914).
SequenceMSIDQRKKMLKNLRNTNYDVFEKICWGLGIEYTFPPLYYRRAHRRFVTKKALCIRVFQETQKLKKRRRALKAAAAAQKQAKRRNPDSPAKAIPKTLKDSQ
Gene ID
Swiss Prot
SynonymsDC37; S15mt; RPMS15; MPR-S15; MRPS15
Calculated MW30kDa
Observed MW28kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesDU145, RD, Cal-27
Cellular locationmitochondrial inner membrane, mitochondrion, nucleolus, nucleoplasm

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - MRPS15 Rabbit mAb (A9388)}

Western blot - MRPS15 Rabbit mAb (A9388)

Western blot analysis of various lysates, using MRPS15 Rabbit mAb (A9388) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.
ABclonal:Immunohistochemistry - MRPS15 Rabbit mAb (A9388)}

Immunohistochemistry - MRPS15 Rabbit mAb (A9388)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MRPS15 Rabbit mAb (A9388) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MRPS15 Rabbit mAb (A9388)}

Immunohistochemistry - MRPS15 Rabbit mAb (A9388)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using MRPS15 Rabbit mAb (A9388) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - MRPS15 Rabbit mAb (A9388)}

Immunofluorescence - MRPS15 Rabbit mAb (A9388)

Immunofluorescence analysis of U2OS cells using MRPS15 Rabbit mAb (A9388) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)