제품 > 항체 > 다중클론항체(pAb)

MRPS36 Rabbit pAb (A24757)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - MRPS36 Rabbit pAb (A24757)

Western blot analysis of lysates from A549 cells using MRPS36 Rabbit pAb(A24757) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - MRPS36 Rabbit pAb (A24757)

Western blot analysis of lysates from Rat heart using MRPS36 Rabbit pAb(A24757) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:5s.

ABclonal:Immunohistochemistry - MRPS36 Rabbit pAb (A24757)

Immunohistochemistry analysis of MRPS36 in paraffin-embedded human colon tissue using MRPS36 Rabbit pAb (A24757) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameMRPS36 Rabbit pAb
Catalog No.A24757
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. The mitochondrial ribosome (mitoribosome) consists of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Pseudogenes corresponding to this gene are found on chromosomes 3p, 4q, 8p, 11q, 12q, and 20p.
ImmunogenRecombinant Protein corresponding to a sequence within amino acids 1-103 of human MRPS36(NP_150597.1).
SequenceMMGSKMASASRVVQVVKPHTPLIRFPDRRDNPKPNVSEALRSAGLPSHSSVISQHSKGSKSPDLLMYQGPPDTAEIIKTLPQKYRRKLVSQEEMEFIQRGGPE
Gene ID
Swiss Prot
SynonymsMRPS36; DC47; MRP-S36; mitochondrial ribosomal protein S36
Calculated MW11kDa
Observed MW14kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesA549, Rat heart
Cellular locationMitochondrion

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - MRPS36 Rabbit pAb (A24757)}

    Western blot - MRPS36 Rabbit pAb (A24757)

    Western blot analysis of lysates from A549 cells using MRPS36 Rabbit pAb(A24757) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Western blot - MRPS36 Rabbit pAb (A24757)}

    Western blot - MRPS36 Rabbit pAb (A24757)

    Western blot analysis of lysates from Rat heart using MRPS36 Rabbit pAb(A24757) at 1:1000 dilution.
    Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection:ECL Basic Kit (RM00020).
    Exposuretime:5s.
    ABclonal:Immunohistochemistry - MRPS36 Rabbit pAb (A24757)}

    Immunohistochemistry - MRPS36 Rabbit pAb (A24757)

    Immunohistochemistry analysis of MRPS36 in paraffin-embedded human colon tissue using MRPS36 Rabbit pAb (A24757) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)