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MSRA Rabbit pAb (A6389)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MSRA Rabbit pAb (A6389)

Western blot analysis of various lysates using MSRA Rabbit pAb (A6389) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - MSRA Rabbit pAb (A6389)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using MSRA Rabbit pAb (A6389) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - MSRA Rabbit pAb (A6389)

Immunofluorescence analysis of NIH/3T3 cells using MSRA Rabbit pAb (A6389) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MSRA Rabbit pAb (A6389)

Immunofluorescence analysis of PC-12 cells using MSRA Rabbit pAb (A6389) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MSRA Rabbit pAb (A6389)

Immunofluorescence analysis of U2OS cells using MSRA Rabbit pAb (A6389) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMSRA Rabbit pAb
Catalog No.A6389
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a ubiquitous and highly conserved protein that carries out the enzymatic reduction of methionine sulfoxide to methionine. Human and animal studies have shown the highest levels of expression in kidney and nervous tissue. The protein functions in the repair of oxidatively damaged proteins to restore biological activity. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-235 of human MSRA (NP_036463.1).
SequenceGNSASNIVSPQEALPGRKEQTPVAAKHHVNGNRTVEPFPEGTQMAVFGMGCFWGAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSAKQMEAALSSKENYQKVLSEHGFGPITTDIREGQTFYYAEDYHQQYLSKNPNGYCGLGGTGVSCPVGIKK
Gene ID
Swiss Prot
SynonymsPMSR; MSRA
Calculated MW26kDa
Observed MW26kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHT-29, SW620, HeLa, Mouse liver, Mouse kidney, Mouse heart, Rat kidney, Rat liver
Cellular locationCytoplasm, Lipid-anchor, Membrane, Mitochondrion, Nucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - MSRA Rabbit pAb (A6389)}

Western blot - MSRA Rabbit pAb (A6389)

Western blot analysis of various lysates using MSRA Rabbit pAb (A6389) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - MSRA Rabbit pAb (A6389)}

Immunohistochemistry - MSRA Rabbit pAb (A6389)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using MSRA Rabbit pAb (A6389) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - MSRA Rabbit pAb (A6389)}

Immunofluorescence - MSRA Rabbit pAb (A6389)

Immunofluorescence analysis of NIH/3T3 cells using MSRA Rabbit pAb (A6389) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MSRA Rabbit pAb (A6389)}

Immunofluorescence - MSRA Rabbit pAb (A6389)

Immunofluorescence analysis of PC-12 cells using MSRA Rabbit pAb (A6389) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MSRA Rabbit pAb (A6389)}

Immunofluorescence - MSRA Rabbit pAb (A6389)

Immunofluorescence analysis of U2OS cells using MSRA Rabbit pAb (A6389) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)