제품 > 항체 > 다중클론항체(pAb)

MT-CO3 Rabbit pAb (A17891)

Publication (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MT-CO3 Rabbit pAb (A17891)

Western blot analysis of lysates from Mouse kidney, using MT-CO3 Rabbit pAb (A17891) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameMT-CO3 Rabbit pAb
Catalog No.A17891
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable electron transfer activity and oxidoreduction-driven active transmembrane transporter activity. Involved in respiratory chain complex IV assembly. Part of respiratory chain complex IV. Implicated in MELAS syndrome.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human MT-CO3 (YP_003024032.1).
SequenceMTHQSHAYHMVKPSPWPLTGALSALLMTSGLAMWFHFHSMTLLMLGLLTNTLTMYQWWRDVTRESTYQGHHTPPVQKGLRYGMILFITSEVFFFAGFFWA
Gene ID
Swiss Prot
SynonymsCOIII; MTCO3; COX3; MT-CO3
Calculated MW30kDa
Observed MW30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse kidney
Cellular locationmitochondrial inner membrane, mitochondrial respiratory chain complex IV, mitochondrion
Customer validation

WB(Homo sapiens, Mus musculus, Cyprinus carpio)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - MT-CO3 Rabbit pAb (A17891)}

Western blot - MT-CO3 Rabbit pAb (A17891)

Western blot analysis of lysates from Mouse kidney, using MT-CO3 Rabbit pAb (A17891) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A17891? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)