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MT-ND1 Rabbit pAb (A5250)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - MT-ND1 Rabbit pAb (A5250)

Western blot analysis of various lysates using MT-ND1 Rabbit pAb (A5250) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameMT-ND1 Rabbit pAb
Catalog No.A5250
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable triplet codon-amino acid adaptor activity. Predicted to act upstream of or within translation. Predicted to be located in mitochondrion. Orthologous to human MT-TI (mitochondrially encoded tRNA-Ile (AUU/C)).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse MT-ND1 (NP_904328.1).
SequenceMFFINILTLLVPILIAMAFLTLVERKILGYMQLRKGPNIVGPYGILQPFADAMKLFMKEPMRPLTTSMSLFIIAPTLSLTLALSLWVPLPMPHPLINLNL
Gene ID
Swiss Prot
SynonymsTrnI; MT-ND1
Calculated MW36kDa
Observed MW36kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, HeLa
Cellular locationMitochondrion inner membrane, Multi-pass membrane protein
Customer validation

WB(Homo sapiens, Mus musculus)

Co-IP(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - MT-ND1 Rabbit pAb (A5250)}

    Western blot - MT-ND1 Rabbit pAb (A5250)

    Western blot analysis of various lysates using MT-ND1 Rabbit pAb (A5250) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A5250? Please let us know so that we can cite the reference in this datasheet.