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MYL2 Rabbit mAb (A8742)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - MYL2 Rabbit mAb (A8742)

Western blot analysis of various lysates using MYL2 Rabbit mAb (A8742) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - MYL2 Rabbit mAb (A8742)

Immunofluorescence analysis of paraffin-embedded rat heart using MYL2 Rabbit mAb (A8742) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MYL2 Rabbit mAb (A8742)

Immunofluorescence analysis of paraffin-embedded mouse heart using MYL2 Rabbit mAb (A8742) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMYL2 Rabbit mAb
Catalog No.A8742
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1286
This gene encodes a major sarcomeric protein in mammalian striated muscle. The encoded protein plays a role in embryonic heart muscle structure and function, while phosphorylation of the encoded protein is involved in cardiac myosin cycling kinetics, torsion and function in adults. Mutations in this gene are associated with hypertrophic cardiomyopathy 10 and infant-onset myopathy.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 67-166 of human MYL2 (P10916).
SequenceDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD
Gene ID
Swiss Prot
SynonymsMLC2; CMH10; MFM12; MLC-2; MLC-2v; MLC-2s/v; MYL2
Calculated MW19kDa
Observed MW19kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IF/ICC 1:100 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse heart, Rat skeletal muscle, Rat heart
Cellular locationA band, Cytoplasm, Myofibril, Sarcomere.

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ABclonal:Western blot - MYL2 Rabbit mAb (A8742)}

Western blot - MYL2 Rabbit mAb (A8742)

Western blot analysis of various lysates using MYL2 Rabbit mAb (A8742) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - MYL2 Rabbit mAb (A8742)}

Immunofluorescence - MYL2 Rabbit mAb (A8742)

Immunofluorescence analysis of paraffin-embedded rat heart using MYL2 Rabbit mAb (A8742) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MYL2 Rabbit mAb (A8742)}

Immunofluorescence - MYL2 Rabbit mAb (A8742)

Immunofluorescence analysis of paraffin-embedded mouse heart using MYL2 Rabbit mAb (A8742) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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