제품 > 항체 > 다중클론항체(pAb)

MYO10 Rabbit pAb (A12471)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of extracts of various cell lines, using MYO10 antibody (A12471) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 20s.

ABclonal:Immunofluorescence - MYO10 Rabbit pAb (A12471)

Immunofluorescence analysis of U2OS cells using MYO10 antibody (A12471) at dilution of 1:350. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of A-549 cells using MYO10 antibody (A12471) at dilution of 1:350. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using MYO10 antibody (A12471) at dilution of 1:350. Blue: DAPI for nuclear staining.

Overview

Product name	MYO10 Rabbit pAb
Catalog No.	A12471
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the myosin superfamily. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-10 (MYH10). Unconventional myosins contain the basic domains of conventional myosins and are further distinguished from class members by their tail domains. This gene functions as an actin-based molecular motor and plays a role in integration of F-actin and microtubule cytoskeletons during meiosis.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 845-944 of human MYO10 (NP_036466.2).
Sequence	EAELRAQQEEETRKQQELEALQKSQKEAELTRELEKQKENKQVEEILRLEKEIEDLQRMKEQQELSLTEASLQKLQERRDQELRRLEEEACRAAQEFLES
Gene ID
Swiss Prot
Synonyms	MyoX
Calculated MW	237kDa
Observed MW	270kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	U-87MG, Mouse kidney, Mouse lung
Cellular location	Cell projection, Cytoplasm, Peripheral membrane protein, cell cortex, cytoskeleton, cytosol, filopodium membrane, filopodium tip, lamellipodium, ruffle
Customer validation	WB(Mus musculus)