제품 > 항체 > 다중클론항체(pAb)

MYO5B Rabbit pAb (A8558)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - MYO5B Rabbit pAb (A8558)

Western blot analysis of extracts of various cell lines, using MYO5B antibody (A8558) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunofluorescence - MYO5B Rabbit pAb (A8558)

Immunofluorescence analysis of Human placenta using MYO5B antibody (A8558) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MYO5B Rabbit pAb (A8558)

Immunofluorescence analysis of Human colon carcinoma using MYO5B antibody (A8558) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameMYO5B Rabbit pAb
Catalog No.A8558
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene, together with other proteins, may be involved in plasma membrane recycling. Mutations in this gene are associated with microvillous inclusion disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1440-1690 of human MYO5B (NP_001073936.1).
SequenceAQALAQSERKRHELNRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTDLKPQMLSGTVPCLPAYILYMCIRHADYTNDDLKVHSLLTSTINGIKKVLKKHNDDFEMTSFWLSNTCRLLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGVLQPMIVSAMLENESIQGLSGVKPTGYRKRSSSMADGDNSYCLEAIIRQMNAFHTVMCDQGLDPEIILQVFKQLFYMIN
Gene ID
Swiss Prot
SynonymsDIAR2; MVID1; PFIC10
Calculated MW214kDa
Observed MW213kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSKOV3, SW480
Cellular locationCytoplasm

Documents

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ABclonal:Western blot - MYO5B Rabbit pAb (A8558)}

Western blot - MYO5B Rabbit pAb (A8558)

Western blot analysis of extracts of various cell lines, using MYO5B antibody (A8558) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunofluorescence - MYO5B Rabbit pAb (A8558)}

Immunofluorescence - MYO5B Rabbit pAb (A8558)

Immunofluorescence analysis of Human placenta using MYO5B antibody (A8558) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MYO5B Rabbit pAb (A8558)}

Immunofluorescence - MYO5B Rabbit pAb (A8558)

Immunofluorescence analysis of Human colon carcinoma using MYO5B antibody (A8558) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)