제품 > 항체 > 다중클론항체(pAb)

MZF1 Rabbit pAb (A10356)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MZF1 Rabbit pAb (A10356)

Western blot analysis of various lysates using MZF1 Rabbit pAb (A10356) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product nameMZF1 Rabbit pAb
Catalog No.A10356
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and protein homodimerization activity. Involved in negative regulation of transcription by RNA polymerase II and positive regulation of transcription by RNA polymerase II. Located in nucleoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-350 of human MZF1 (NP_003413.2).
SequenceIQARVQGQRPGSPEEAAALVDGLRREPGGPRRWVTVQVQGQEVLSEKMEPSSFQPLPETEPPTPEPGPKTPPRTMQESPLGLQVKEESEVTEDSDFLESGPLAATQESVPTLLPEEAQRCGTVLDQIFPHSKTGPEGPSWREHPRALWHEEAGGIFSPGFALQLGSISAGPGSVSPHLHVPWDLGMAGLSGQIQSPSREGGFAHALLLPSDLRSEQDPTDEDPCRGVGPALITTRWRSPRGRSRGRPSTGG
Gene ID
Swiss Prot
SynonymsMZF-1; MZF1B; ZFP98; ZNF42; ZSCAN6; MZF1
Calculated MW82kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, A-431, U-251MG, 293T, SGC-7901, Mouse brain, Mouse lung, Rat brain
Cellular locationNucleus
Customer validation

WB(Mus musculus, Rattus norvegicus)

IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - MZF1 Rabbit pAb (A10356)}

Western blot - MZF1 Rabbit pAb (A10356)

Western blot analysis of various lysates using MZF1 Rabbit pAb (A10356) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A10356? Please let us know so that we can cite the reference in this datasheet.