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Mast Cell Tryptase (TPSB2) Rabbit pAb (A15887)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - Mast Cell Tryptase (TPSB2) Rabbit pAb (A15887)

Western blot analysis of various lysates using Mast Cell Tryptase (TPSB2) Rabbit pAb (A15887) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameMast Cell Tryptase (TPSB2) Rabbit pAb
Catalog No.A15887
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human Mast Cell Tryptase (Mast Cell Tryptase (TPSB2)) (NP_077078.5).
SequenceMLNLLLLALPVLASRAYAAPAPGQALQRVGIVGGQEAPRSKWPWQVSLRVRDRYWMHFCGGSLIHPQWVLTAAHCVGPDVKDLAALRVQLREQHLYYQDQLLPVSRIIVHPQFYTAQIGADIALLELEEPVNVSSHVHTVTLPPASETFPPGMPCWVTGWGDVDNDERLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIVRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP
Gene ID
Swiss Prot
SynonymsTPS2; tryptaseB; tryptaseC; Mast Cell Tryptase (TPSB2)
Calculated MW31kDa
Observed MW30kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesH-460, Rat heart
Cellular locationSecreted
    ABclonal:Western blot - Mast Cell Tryptase (TPSB2) Rabbit pAb (A15887)}

    Western blot - Mast Cell Tryptase (TPSB2) Rabbit pAb (A15887)

    Western blot analysis of various lysates using Mast Cell Tryptase (TPSB2) Rabbit pAb (A15887) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (1)

    Secondary Antibodies (26)