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Matrin 3 Rabbit pAb (A5905)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Matrin 3 Rabbit pAb (A5905)

Western blot analysis of various lysates using Matrin 3 Rabbit pAb (A5905) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Matrin 3 Rabbit pAb (A5905)

Immunohistochemistry analysis of paraffin-embedded Rat testis using Matrin 3 Rabbit pAb (A5905) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Matrin 3 Rabbit pAb (A5905)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using Matrin 3 Rabbit pAb (A5905) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Matrin 3 Rabbit pAb (A5905)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Matrin 3 Rabbit pAb (A5905) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Matrin 3 Rabbit pAb (A5905)

Immunofluorescence analysis of C6 cells using Matrin 3 Rabbit pAb (A5905) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Matrin 3 Rabbit pAb (A5905)

Immunofluorescence analysis of NIH/3T3 cells using Matrin 3 Rabbit pAb (A5905) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Matrin 3 Rabbit pAb (A5905)

Immunofluorescence analysis of U-2 OS cells using Matrin 3 Rabbit pAb (A5905) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMatrin 3 Rabbit pAb
Catalog No.A5905
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a nuclear matrix protein, which is proposed to stabilize certain messenger RNA species. Mutations of this gene are associated with distal myopathy 2, which often includes vocal cord and pharyngeal weakness. Alternatively spliced transcript variants, including read-through transcripts composed of the upstream small nucleolar RNA host gene 4 (non-protein coding) and matrin 3 gene sequence, have been identified. Pseudogenes of this gene are located on chromosomes 1 and X.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 747-847 of human Matrin 3 (NP_001181884.1).
SequenceSSENADDPNKDTSENADGQSDENKDDYTIPDEYRIGPYQPNVPVGIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKKET
Gene ID
Swiss Prot
SynonymsMPD2; ALS21; VCPDM; Matrin 3
Calculated MW95kDa
Observed MW125kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HepG2, MCF7, K-562, NIH/3T3
Cellular locationNucleus matrix

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ABclonal:Western blot - Matrin 3 Rabbit pAb (A5905)}

Western blot - Matrin 3 Rabbit pAb (A5905)

Western blot analysis of various lysates using Matrin 3 Rabbit pAb (A5905) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Matrin 3 Rabbit pAb (A5905)}

Immunohistochemistry - Matrin 3 Rabbit pAb (A5905)

Immunohistochemistry analysis of paraffin-embedded Rat testis using Matrin 3 Rabbit pAb (A5905) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Matrin 3 Rabbit pAb (A5905)}

Immunohistochemistry - Matrin 3 Rabbit pAb (A5905)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using Matrin 3 Rabbit pAb (A5905) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Matrin 3 Rabbit pAb (A5905)}

Immunohistochemistry - Matrin 3 Rabbit pAb (A5905)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Matrin 3 Rabbit pAb (A5905) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Matrin 3 Rabbit pAb (A5905)}

Immunofluorescence - Matrin 3 Rabbit pAb (A5905)

Immunofluorescence analysis of C6 cells using Matrin 3 Rabbit pAb (A5905) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Matrin 3 Rabbit pAb (A5905)}

Immunofluorescence - Matrin 3 Rabbit pAb (A5905)

Immunofluorescence analysis of NIH/3T3 cells using Matrin 3 Rabbit pAb (A5905) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Matrin 3 Rabbit pAb (A5905)}

Immunofluorescence - Matrin 3 Rabbit pAb (A5905)

Immunofluorescence analysis of U-2 OS cells using Matrin 3 Rabbit pAb (A5905) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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