제품 > 항체 > 다중클론항체(pAb)

Med25 Rabbit pAb (A21274)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Arabidopsis thaliana

ABclonal:Western blot - Med25 Rabbit pAb (A21274)

Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using Med25 Rabbit pAb (A21274) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameMed25 Rabbit pAb
Catalog No.A21274
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Encodes a nuclear protein that acts in a phyB pathway (but downstream of phyB) and induces flowering in response to suboptimal light conditions. PFT1 promotes flowering in CO dependent and independent pathways and integrates several environmental stimuli, such as light quality and JA-dependent defenses. Mutants are hypo-responsive to far-red and hyper-responsive to red light and flower late under long day conditions. Also shown to be a Mediator subunit regulating jasmonate-dependent defense.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of arabidopsis thaliana Med25 (NP_173925.3).
SequenceAGKPNQQSADLSIDTAKNTFYLVLISENFVEACAALSHSATNLPQTQSPVKVDRATVAPSIPVTGQPPAPVSSANGPIQNRQPVSVGPVPTATVKVEPSTV
Gene ID
Swiss Prot
SynonymsF2J7.4; F2J7_4; MED25; mediator 25; PHYTOCHROME AND FLOWERING TIME 1; Med25
Calculated MW90kDa
Observed MW75kDa
ReactivityArabidopsis thaliana
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSeedling, Inflorescences, Before inflorescence, Rosette leaf
Cellular locationnucleus
    ABclonal:Western blot - Med25 Rabbit pAb (A21274)}

    Western blot - Med25 Rabbit pAb (A21274)

    Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using Med25 Rabbit pAb (A21274) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.