제품 > 항체 > 다중클론항체(pAb)

Mfsd2a Rabbit pAb (A18288)

Publication (0) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Mfsd2a Rabbit pAb (A18288)

Western blot analysis of various lysates using Mfsd2a Rabbit pAb (A18288) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

Overview

Product nameMfsd2a Rabbit pAb
Catalog No.A18288
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables fatty acid transmembrane transporter activity; lysophosphatidylcholine flippase activity; and lysophospholipid:sodium symporter activity. Involved in central nervous system development; lipid transport; and transcytosis. Acts upstream of or within several processes, including photoreceptor cell development; regulation of lipid metabolic process; and retina development in camera-type eye. Located in plasma membrane. Is integral component of plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; integumental system; and sensory organ. Human ortholog(s) of this gene implicated in primary autosomal recessive microcephaly 15. Orthologous to human MFSD2A (major facilitator superfamily domain containing 2A).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 170-245 of mouse Mfsd2a (NP_083938.2).
SequenceSALTMFISTEQSERDSATAYRMTVEVLGTVIGTAIQGQIVGQAKAPCLQDQNGSVVVSEVANRTQSTASLKDTQNA
Gene ID
Swiss Prot
SynonymsNLS1; Mfsd2; 1700018O18Rik; Mfsd2a
Calculated MW59kDa
Observed MW60-70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2, Mouse brain, Rat brain
Cellular locationplasma membrane.

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    ABclonal:Western blot - Mfsd2a Rabbit pAb (A18288)}

    Western blot - Mfsd2a Rabbit pAb (A18288)

    Western blot analysis of various lysates using Mfsd2a Rabbit pAb (A18288) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 120s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)