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Mrpl46 Rabbit pAb (A18187)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Mrpl46 Rabbit pAb (A18187)

Western blot analysis of various lysates using Mrpl46 Rabbit pAb (A18187) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Mrpl46 Rabbit pAb (A18187)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Mrpl46 Rabbit pAb (A18187) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameMrpl46 Rabbit pAb
Catalog No.A18187
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be a structural constituent of ribosome. Located in mitochondrion. Is expressed in several structures, including early conceptus; gonad; heart; liver; and metanephros. Orthologous to human MRPL46 (mitochondrial ribosomal protein L46).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-283 of mouse Mrpl46 (NP_075820.1).
SequenceMAAPVGRTLLGLARGWRQLDRFWAGSSRGLSLEAASSSSRSPWRLSGALCLQRPPLITKALTPLQEEMAGLLQQIEVERSLYSDHELRALDEAQRLAKKKADLYDEEQEQGITLAQDLEDMWEQAFLQFRPGARETEADKKNDRTSLHRKLDRNLVLLVREKLGDQDVWMLPQVEWQPGETLRGTAERILATLSENNMEAKFLGNAPCGHYKFKFPKAIQTESDLGVKVFFFKALLLTGDFVQAGKKSRHVWASKEELGDYLQPKYLAQVRRFLLDSDGLSCL
Gene ID
Swiss Prot
SynonymsLIECG2; P2ECSL; C15orf4; 3110052F15Rik; Mrpl46
Calculated MW32kDa
Observed MW32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-251MG, A-549, HepG2, U2OS, Mouse testis, Mouse liver, Rat thymus, Rat uterus
Cellular locationmitochondrial inner membrane, mitochondrion, nucleoplasm

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Mrpl46 Rabbit pAb (A18187)}

    Western blot - Mrpl46 Rabbit pAb (A18187)

    Western blot analysis of various lysates using Mrpl46 Rabbit pAb (A18187) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - Mrpl46 Rabbit pAb (A18187)}

    Immunohistochemistry - Mrpl46 Rabbit pAb (A18187)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Mrpl46 Rabbit pAb (A18187) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.