제품 > 항체 > 다중클론항체(pAb)

Mrpl48 Rabbit pAb (A18188)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Mrpl48 Rabbit pAb (A18188)

Western blot analysis of various lysates using Mrpl48 Rabbit pAb (A18188) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameMrpl48 Rabbit pAb
Catalog No.A18188
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Located in mitochondrion. Is expressed in several structures, including early conceptus; gonad; heart; liver; and metanephros. Orthologous to human MRPL48 (mitochondrial ribosomal protein L48).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 28-211 of mouse Mrpl48 (NP_942128.2).
SequenceSLGENPIFSAGGILWTSRHYKTKPTRGIGRYRHLVKVQEPKKKKAKVELRAINVGTDYEYGVLNIHLTAYDMSLAESYAQYVHRLCNRLSIKVEESYAMPTKTMEVMRLPDQGNKMVLDSILTTHERVVQISGLSATFAEIFLEVLQSNLPEGVRLSVREHTEEDFKGRFKARPELEELLAKLN
Gene ID
Swiss Prot
SynonymsL48mt; CGI-118; MRP-L48; D4Ertd786e; 1810030E20Rik; 2610028L11Rik; Mrpl48
Calculated MW24kDa
Observed MW19kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7, Mouse liver, Mouse spleen, Rat brain
Cellular locationmitochondrial inner membrane, mitochondrial ribosome, mitochondrion
    ABclonal:Western blot - Mrpl48 Rabbit pAb (A18188)}

    Western blot - Mrpl48 Rabbit pAb (A18188)

    Western blot analysis of various lysates using Mrpl48 Rabbit pAb (A18188) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)