제품 > 항체 > 다중클론항체(pAb)

MtLBs Rabbit pAb (A20191)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Medicago trucatula

Western blot analysis of lysates from roots from Medicago trucatula, using MtLBs Rabbit pAb (A20191) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 30s.

Overview

Product name	MtLBs Rabbit pAb
Catalog No.	A20191
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Provides oxygen to the bacteroids. This role is essential for symbiotic nitrogen fixation.

Immunogen information

Immunogen	Two synthetic peptides corresponding to the sequence within amino acids 7-21 and 100-113 of Medicago trucatula Leghemoglobin 2 (XP_003588689.1).
Sequence	DRHFMVVKEALLKT/QEALVNSSWELFKQN
Gene ID
Swiss Prot
Synonyms	LB2; MTlb2; MtLBs
Calculated MW	15kDa
Observed MW	15kDa

Applications

Reactivity	Medicago trucatula
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	roots
Cellular location
Customer validation	WB(M. truncatula)