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Mu Opioid Receptor(MOR) Rabbit pAb (A16939)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Mu Opioid Receptor(MOR) Rabbit pAb (A16939) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Mu Opioid Receptor(MOR) Rabbit pAb (A16939)

Immunohistochemistry analysis of paraffin-embedded Rat brain using Mu Opioid Receptor(MOR) Rabbit pAb (A16939) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Mu Opioid Receptor(MOR) Rabbit pAb (A16939)

Immunofluorescence analysis of paraffin-embedded mouse brain using Mu Opioid Receptor(MOR) Rabbit pAb (A16939) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Mu Opioid Receptor(MOR) Rabbit pAb
Catalog No.	A16939
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes one of at least three opioid receptors in humans; the mu opioid receptor (MOR). The MOR is the principal target of endogenous opioid peptides and opioid analgesic agents such as beta-endorphin and enkephalins. The MOR also has an important role in dependence to other drugs of abuse, such as nicotine, cocaine, and alcohol via its modulation of the dopamine system. The NM_001008503.2:c.118A>G allele has been associated with opioid and alcohol addiction and variations in pain sensitivity but evidence for it having a causal role is conflicting. Multiple transcript variants encoding different isoforms have been found for this gene. Though the canonical MOR belongs to the superfamily of 7-transmembrane-spanning G-protein-coupled receptors some isoforms of this gene have only 6 transmembrane domains.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 354-388 of human Mu Opioid Receptor(MOR) (NP_000905.3).
Sequence	IPTSSNIEQQNSTRIRQNTRDHPSTANTVDRTNHQ
Gene ID
Swiss Prot
Synonyms	MOP; MOR; LMOR; MOR1; OPRM; M-OR-1; Mu Opioid Receptor(MOR)
Calculated MW	45kDa
Observed MW	Refer to figures

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples
Cellular location	Cell membrane, Cytoplasm, Multi-pass membrane protein.