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Musashi-1 (MSI1) Rabbit mAb (A9122)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Musashi-1 (MSI1) Rabbit mAb (A9122)

Western blot analysis of extracts of various cell lines, using Musashi-1 (MSI1) Rabbit mAb (A9122) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunofluorescence - Musashi-1 (MSI1) Rabbit mAb (A9122)

Immunofluorescence analysis of NIH-3T3 cells using Musashi-1 (MSI1) Rabbit mAb (A9122) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Musashi-1 (MSI1) Rabbit mAb (A9122)

Immunofluorescence analysis of U-2 OS cells using Musashi-1 (MSI1) Rabbit mAb (A9122) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameMusashi-1 (MSI1) Rabbit mAb
Catalog No.A9122
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1796
This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human MSI1 (O43347).
SequenceMETDAPQPGLASPDSPHDPCKMFIGGLSWQTTQEGLREYFGQFGEVKECLVMRDPLTKRSRGFGFVTFMDQAGVDKVLAQSRHELDSKTIDPKVAFPRRA
Gene ID
Swiss Prot
SynonymsMSI1; musashi RNA binding protein 1
Calculated MW39kDa
Observed MW39kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSH-SY5Y, Mouse brain
Cellular locationCytoplasm, Nucleus

Documents

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ABclonal:Western blot - Musashi-1 (MSI1) Rabbit mAb (A9122)}

Western blot - Musashi-1 (MSI1) Rabbit mAb (A9122)

Western blot analysis of extracts of various cell lines, using Musashi-1 (MSI1) Rabbit mAb (A9122) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunofluorescence - Musashi-1 (MSI1) Rabbit mAb (A9122)}

Immunofluorescence - Musashi-1 (MSI1) Rabbit mAb (A9122)

Immunofluorescence analysis of NIH-3T3 cells using Musashi-1 (MSI1) Rabbit mAb (A9122) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Musashi-1 (MSI1) Rabbit mAb (A9122)}

Immunofluorescence - Musashi-1 (MSI1) Rabbit mAb (A9122)

Immunofluorescence analysis of U-2 OS cells using Musashi-1 (MSI1) Rabbit mAb (A9122) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)